关键词:   
Adenine base editors catalyze cytosine conversions in human cells
Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5-7) and in a confined TC*N sequence context. Adenine base editor-induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing.
pmid: 31548727 Nat Biotechnol 影响因子: 31.864 发表日期: 20190923 官网 免费下载
文献:

评论:

    菠萝视频下载安装